American Society of Hematology

Figure 5

$Figure 5. The KPT-185/Nutlin-3a combination induces p53-mediated cell death. (A) OCI-AML-3 cells expressing control shRNA (shC) or p53-specific shRNA (shp53) were treated with the indicated concentrations of KPT-185 or Nutlin-3a for 72 hours, either as individual agents or in combination. Cell viability was determined by trypan blue exclusion method. Annexin V–positive fractions were measured by flow cytometry. (B) MOLM-13 and MV4;11 cells expressing control shRNA (shC) or p53-specific shRNA (shp53) were treated with the indicated concentrations of KPT-185 or Nutlin-3a for 72 hours, either as individual agents or in combination. Cell viability was determined by trypan blue exclusion method. Comparable results were obtained in 2 other independent experiments.$

The KPT-185/Nutlin-3a combination induces p53-mediated cell death. (A) OCI-AML-3 cells expressing control shRNA (shC) or p53-specific shRNA (shp53) were treated with the indicated concentrations of KPT-185 or Nutlin-3a for 72 hours, either as individual agents or in combination. Cell viability was determined by trypan blue exclusion method. Annexin V–positive fractions were measured by flow cytometry. (B) MOLM-13 and MV4;11 cells expressing control shRNA (shC) or p53-specific shRNA (shp53) were treated with the indicated concentrations of KPT-185 or Nutlin-3a for 72 hours, either as individual agents or in combination. Cell viability was determined by trypan blue exclusion method. Comparable results were obtained in 2 other independent experiments.

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