The KPT-185/Nutlin-3a combination enhances nuclear function of p53. (A) The KPT-185/Nutlin-3a combination accumulates p53 into the nucleus of AML cells. OCI-AML-3 cells were treated for 6 hours with 100 nM KPT-185 and 2.5 µM Nutlin-3a. Cells were stained for p53 (green) and mitochondrial marker protein cytochrome c oxidase IV (COX-IV, red) and visualized by confocal microscopy. Nuclei were counterstained with 4′,6-diamidino-2-phenylindole(blue). (B) Expression of p53 target proteins in OCI-AML-3 cells, which were treated with 50 nM KPT-185 and/or 2.5 µM Nutlin-3a for 12 hours. Intensity of the immunoblot signals was quantified, and the relative intensity compared with β-actin was calculated. Results are representative of 3 independent experiments.