Tat association with the IRF7, MAP2K6, and MAP2K3 promoters in primary iDC and MDM. (A) Signals obtained by PCR in iDC expressing wild type TatSF2 or the mutant TatSF2G48R57A with two or three sets of primers carried out with input DNA (90, 30, 10 ng) or with 3 ng of DNA extracted from immunoprecipitated samples are shown. (B) The average fold enrichment of the IRF7, MAP2K3, and MAP2K6 promoter in primary iDC in the immunoprecipitated DNA relative to input DNA +/− SEM from three independent qPCR experiments is reported. All Ct values obtained with immunoprecipitated DNAs were compared with the Ct value obtained with the same amount of the corresponding input DNA. (C) Luciferase activity of lysates from primary iDC expressing no Tat (tTA), wild type TatSF2, TatSF2 G48R57A and transfected with an IRF7- or MAP2K6-luciferase reporter vector. (D,E) Enrichments of the IRF7, MAP2K3, and MAP2K6 promoters after Tat immunoprecipitation from chromatin lysates of primary iDC infected with a replication competent HIVSF2, expressing a flagged Tat. (F) Signals obtained by PCR in MDM expressing wild type TatSF2 or the mutant TatSF2G48R57A with two or three sets of primers carried out with input DNA (90, 30, 10 ng) or with 3 ng of DNA extracted from immunoprecipitated samples are shown. (G) The average fold enrichment of the IRF7, MAP2K3, and MAP2K6 promoter in primary MDM in the immunoprecipitated DNA relative to input DNA +/− SEM from three independent qPCR experiments is reported. (H,I) Enrichments of the IRF7, MAP2K3, and MAP2K6 promoters after Tat immunoprecipitation from chromatin lysates of MDM infected with a replication competent HIVSF2, expressing a flagged Tat.