aUPD in chromosome 3p in 4 patients with WM. (A) Representative excerpts from paired tumor and germline tissue documenting an aUPD on chromosome 3 for a single WM patient. The red histogram below the ideogram corresponds to the heterozygous call rate. Below that, copy number (black line) and normalized relative coverage (orange) is shown above the LAF measurements (blue). LAF values more than 2 standard deviations from the mean are shown in green. (B) Sanger sequencing of MYD88 L265P for this patient with a near homozygous mutant signal. (C) Heterozygous call rate, LAF, and relative coverage for the 4 patients with aUPDs in chromosome 3p demonstrating the near identical location of the aUPD for the 4 affected patients.