Figure 1
The binding of various concentrations of recombinant FXIII-A2to FXIII-B2. (A) FXIII-B prepared from plasma was fixed to a CM5 chip and the binding of FXIII-A2 was followed by the measurement of SPR. Each sensorgram corresponds to the rFXIII-A2 concentration indicated in the figure. The data were fit to a simple 1:1 interaction model using the global data analysis option available within Biaevaluation software. (B) The binding of rFXIII-A2 to plasma-derived FXIII-B2 was detected in an ELISA-type binding assay; the binding curve represents the means of 3 separate experiments not deviating by more than 10%. FXIII-A2B2/FXIII-B2(t): the molar concentration of measured formed FXIII-A2B2 was divided by molar concentration of total added FXIII-B2.
