Role of PAR-1 in vascular inflammation in SCD. (A-D) Plasma levels of TAT (A), sVCAM-1 (B), IL-6 (C), and MPO (D) in the lung of PAR-1^+/+ or PAR-1^−/− mice transplanted with bone marrow from WT (n = 9-11) or BERK (n = 15-16) mice. Formalin-fixed lungs were stained for neutrophils, and neutrophils were counted in 10 HPF (×400) for each mouse. (E) Average number of neutrophils in lungs. Neutrophils stain brown. Asterisks directly above the bars indicate statistically significant difference between BERK^BM mice compared with WT^BM mice within the same PAR-1 genotype (*P < .05, **P < .01, and ***P < .001). There were no differences between PAR-1^+/+ and PAR-1^−/− mice.