Inhibition of thrombus size in male (NZW × BXSB)F1 mice by A1-A1. In vivo imaging of platelet accumulation at the site of injury in male (NZW × BXSB)F1 mice after laser-induced thrombosis in cremaster muscle arterioles in the absence of A1-A1 and after the infusion of A1-A1 at a dose of 4 μg/g mouse. Data were acquired in 13- to 14-week-old mice (A-C) and in 6-week-old mice (D-E). (A) Platelet accumulation during thrombus formation represented by the median-integrated platelet fluorescence studied in 5 mice in the absence of A1-A1 (black curve, 33 thrombi) and in the presence of A1-A1 (red curve, 32 thrombi). Area under the curve of platelet fluorescence over time is a measure of the thrombus size. In the presence of A1-A1, thrombus size is decreased to <15% of the thrombus size in the absence of A1-A1. (B) Snapshots of platelet accumulation at the site of injury over time. Comparison of 2 representative thrombi generated in the same mouse in the absence (top row) and in the presence (bottom row) of A1-A1. (C) Area under the curve of individual thrombi collected in the absence (black circles) and in the presence of A1-A1 (red circles). Inhibition of thrombus size in the presence of A1-A1 is statistically significant (P = .001). (D) Median-integrated platelet fluorescence studied in 5 mice in the absence (black curve, 32 thrombi) and in the presence (red curve, 30 thrombi) of A1-A1. (E) Area under the curve of individual thrombi collected in the absence (black circles) and in the presence of A1-A1 (red circles). The difference between 2 data sets is not statistically significant. Lines on panels C and E correspond to areas under the curves of the median-integrated fluorescence calculated for all thrombi in a data set.
