LA6, which does not bind β2GPI, does not inhibit thrombus size in (NZW × BXSB)F1mice. In vivo imaging of platelet accumulation at the site of injury in 13- to 14-week-old male (NZW × BXSB)F1 mice after laser-induced thrombosis in cremaster muscle arterioles in the absence of LA6 and after the infusion of LA6 at a dose of 4 μg/g mouse. (A) Median-integrated platelet fluorescence studied in 6 mice in the absence of LA6 (black curve, 37 thrombi) and in the presence of LA6 (red curve, 33 thrombi). (B) Snapshots of platelet accumulation at the site of injury over time. Comparison of 2 representative thrombi generated in the same mouse in the absence (top row) and in the presence (bottom row) of LA6. (C) Sizes of individual thrombi collected in the absence (black circles) and in the presence of LA6 (red circles). Lines correspond to areas under the curves of the median-integrated fluorescence calculated for all thrombi in each data set collected in 6 mice. The difference between 2 data sets is not statistically significant (P = .42).
