BCL6 stabilizes BACH2 protein. (A) Immunoblot analysis of BACH2 and BCL6 protein levels in OCI-LY1 cells treated with siNT or siBCL6 (#1 and #2) for 72 hours. (B) Immunoblot analysis of BACH2 and BCL6 protein levels in OCI-LY1 cells treated with siNT or siBCL6#1 at 3 different time points. (A-B) The relative amount of BACH2 to actin was quantified by densitometry (indicated by numbers below the BACH2 immunoblot). (C) OCI-LY1 cells were treated with siNT or siBACL6#1 for 24 hours, followed by incubation with 5 μM cycloheximide (CHX) for indicated time periods. Immunoblot analysis was performed to determine BCL6 and BACH2 protein levels. The relative amount of BACH2 to actin was quantified by densitometry (Y-axis) and plotted with respect to time (X-axis). The BACH2 to actin level in CHX-untreated cells is calculated as 100%.