Target verification of miR-130a in C/EBP-ε 3ʹ-UTR. HEK293 cells or A549 cells were transfected with pMIR-CEBPE-wt or pMIR-CEBPE-mut and (A) pre-miR-130a/scrambled miRNA or (B) LNA-130a/LNA-neg, respectively. The cells were harvested 24 hours posttransfection and luciferase activity was measured using the Dual-Luciferase Reporter Assay System. The firefly luciferase activity was normalized to the activity of renilla luciferase expressed from pGL4,74 included in each transfection. Error bars show SD from triplicate measurements. The expressions from the transfections with the controls (miR-neg and LNA-neg) were assigned the value 1 and the relative expression measured from the constructs co-transfected with miR-130a or LNA-130a was recalculated accordingly. (C) Real-time PCR measuring the internal miR-130a level in A549 and HEK293 cells. Error bars indicate SD between 3 measurements. Asterisks indicate significant differences in expression between transfections (*P < .05).