The miR-130a affects cell growth and expression of mRNAs encoding specific granule protein. MPRO cells were stably transfected with the vectors pEGP-miR-130a and pEGP-miR-Null. (A) Relative miR-130a expression in the stably transfected clones. (B) Western blot analysis shows C/EBP-ε protein expression in the same cells as in (A). (C) Relative expression level of Cebpe, Ltf, Camp, and Lcn2 mRNAs in the 2 clones. Error bars indicate SD between 3 individual measurements. Asterisks indicate significant differences in expression between the 2 clones (*P < .05). (D) Cell growth of the clones over a 4-day period. (E) Cell cycle profile of the 2 clones. (F) Cytospins showing the clones stained with May-Grünwald Giemsa; scale bars = 20 μm. Cytospins were photographed using an Olympus BX51 microscope (100×/1.35 PlanApo oil objective) with an Olympus DP70 camera and the analySIS B5.0 software package (Olympus). (G) FACS analysis of the surface marker CD11b.