MPRO cells overexpressing miR-130a resists ATRA-induced differentiation. (A) Immunoblot of C/EBP-ε in pEGP-control and pEGP-miR-130a during stimulation with ATRA over a 5-day period. (B) The relative expression level of Ltf, Camp, and Lcn2 mRNAs at day 2 of differentiation in the clones measured by real-time PCR. Error bars indicate SD. (C) Cell cycle profile of the 2 clones at day 0 and day 4 of ATRA stimulation. (D) Cytospins showing the clones on day 4 after ATRA stimulation stained with May-Grünwald Giemsa; scale bars = 20 μm. Photographed as in Figure 4. (E) Flow analysis of surface marker CD11b. Asterisks indicate significant differences in expression between the 2 cell populations (*P < .05).