Suppression of IFN-γ production by Ezh2 requires PRC2. (A) Expression of Ezh2, Ezh1, and Suz12 in naïve and differentiated T cells relative to Rps9. Data are mean ± SEM of 2 independent experiments. P values were calculated from 2-way analysis of variance (*P < .05). (B) Western blot showing Ezh2, Suz12, Ezh1, H3k27me3, and Actb in naïve CD4⁺ T cells isolated from WT (Ly5.1), Ezh2^fl/fl, Ezh2^fl/+CD4Cre, and Ezh2^fl/fl.CD4Cre mice. (C) Western blots of iTreg cells. (D) Western blot of naïve CD4⁺ T cells that are depleted of Suz12. (E) IFN-γ and Foxp3 expression in Suz12-kd Th0 and iTreg cells. (F) Cytokine expression in Suz12-kd Th0 cells.