Schema for manufacture of membrane proteoliposomal vaccine. Following an excisional biopsy of at least a 2 cm lymph node, the tumor sample is processed into a single cell suspension and proteins from whole cell membranes are directly extracted from 2 × 109 lymph node biopsy cells with detergent. The membrane proteins are incorporated into liposomes along with interleukin (IL)-2 to produce membrane-patched proteoliposome vaccine. The liposomes are initially in the form of small unilamellar vesicles (SUV) when synthesized from dimyristoylphosphatidylcholine (DMPC). These SUVs coalesce following incubation with IL-2 to form large multilamellar vesicles with the incorporation of IL-2 and various membrane proteins within the lipid bilayers.
Abbreviations: Id, idiotype; MHC Ag, major histocompatibility antigen complex; B7, T-cell costimulatory molecule.
