Kaplan-Meier survival of WT or Ptch2^-/- C57BL/6 mice transplanted with JAK2V617F-GFP or empty-GFP transduced WT or Ptch2^-/- C57BL/6 BM cells (n≥5 each group). B PB samples were analyzed for cKit⁺ CD11b⁺ myeloid blasts by flow cytometry. Bethesda criteria published 2002 claim 20% blasts as critical value between MPN and AML. C Representative image of the pronounced splenomegaly within the Ptch2^-/- group.