Acalabrutinib pharmacodynamics in ibrutinib-intolerant patients. (A) BTK occupancy for ibrutinib-intolerant patients with day-1 (D1) predose (Pre) signal/noise ratio ≥5 (n = 4 patients excluded for this reason). For the box plots, the horizontal line in the center of the box shows the median, and the upper and lower edges of the box show the 25th and 75th percentiles, respectively. The I bars (whiskers) represent 1.5× the interquartile range, with symbols showing outliers according to the Tukey method. (B) BCR-induced BTK phosphorylation (p) shown as fold over D1 predose plus exogenous acalabrutinib control. Filtered on D1 predose fold change >1.5 (n = 15 patients excluded for this reason; n = 2 patients had insufficient cells to perform the assay). Significance was determined using a paired, 2-tailed, parametric Student t test comparing time points with D1 predose. ***P < .001, ****P < .0001. C, cycle; Post, postdose; SD, standard deviation.