Data showing the time course of glomerular injury in male and female HbSS and HbAA mice. (A) Quantification of WT-1⁺–stained glomerular sections from male HbAA and HbSS mice represented as number of WT-1⁺–stained cells per glomerulus. (B) Relative glomerular podocin expression in glomeruli isolated from male HbAA and HbSS mice. (C) Relative glomerular nephrin expression in glomeruli isolated from male HbAA And HbSS mice. (D) Quantification of WT-1⁺–stained glomerular sections from female HbAA and HbSS mice represented as number of WT-1⁺–stained cells per glomerulus. (E) Relative glomerular podocin expression in glomeruli isolated from female HbAA and HbSS mice. (F) Relative glomerular nephrin expression in glomeruli isolated from female HbAA and HbSS mice. (G) Representative WT-1⁺–stained sections of glomeruli from male and female HbAA and HbSS mice. Original magnification ×40; scale bars = 50 μm. Data are mean ± SEM; *P < .05 vs 8-week HbSS; #P < .05 vs 8-week HbAA. Analysis with 2-way ANOVA with Tukey post hoc analysis. (A) Interaction, genotype, and age, P < .0001. (B) Interaction and age, P < .0001; genotype, P = .4236. (C) Interaction, P = .0028; genotype, P < .0001; age, P = .0739. (D) Interaction and genotype, P < .0001; age, P = .0004. (E) Interaction, P = .2687; genotype, P = .0039; age, P = .0017. (F) Interaction, P = .5020; genotype, P = 9953; age, P = .0411.