Time course in additional measures of renal tubular injury in male and female HbSS mice compared with genetic controls (HbAA). (A) Relative NGAL expression in renal cortex of male HbAA and HbSS mice. (B) Relative NGAL expression in renal cortex of female HbAA and HbSS mice. (C) Relative megalin expression in renal cortex of male HbAA and HbSS mice. (D) Relative megalin expression in renal cortex of female HbAA and HbSS mice. (E) Quantification of iron deposition in the whole kidney scans (represented as megapixels per micrometer) in male HbAA and HbSS mice. Original magnification ×40; scale bar = 50 μm. (F) Quantification of iron deposition in the whole kidney scans (represented as megapixels per micrometer) in male HbAA and HbSS mice. (G) Relative caspase-3 expression in renal cortex of male HbAA and HbSS mice. (H) Relative caspase-3 expression in renal cortex of female HbAA and HbSS mice. (I) Relative HIF-1α expression in renal cortex of male and females HbSS mice. (J) Representative Prussian blue–stained sections of renal cortex from male and female HbAA and HbSS mice. Original magnification ×10; scale bar = 100 μm. Data are mean plus or minus SEM; *P < .05 vs 8-week HbSS mice; **P < .05 vs age-matched males HbSS. Analysis by 2-way ANOVA with the Tukey post hoc test. (A) Interaction, P = .0099; genotype, P < .0001; age, P = .0097. (B) Interaction, P = .0031; genotype, P < .0001; age, P = .0234. (C) Interaction, P = .0176; genotype, P < .0001; age, P = .0184. (D) Interaction, P = .2074; genotype, P = .0119; age, P = .1529. (E) Interaction, P = .0002; genotype, P < .0001; age, P = .0002. (F) Interaction, P = .4335; genotype, P < .0001; age, P = .1189. (G) Interaction, P = .3509; genotype, P < .0001; age, P = .3981. (H) Interaction, P = .0007; genotype, P < .0001; age, P = .7121, or unpaired Student t test (I).