Distribution of human myeloid subsets in BRGSF mice and effect of Flt3L on their development. Representative flow cytometry immunophenotypic analysis of hCD45⁺HLA⁻DR⁺CD19⁻CD3⁻CD56⁻ cells from bone marrow (A) and spleen (C) of an Flt3L-treated mouse and a PBS-treated littermate engrafted with the same CD34⁺ HSC donor. Comparison of frequencies within the human CD45⁺ cells and total number of the 4 myeloid subsets (CD14⁺ monocytes, CD123⁺ pDCs, CD141⁺ cDCs, and CD1c⁺ cDCs) with or without Flt3L treatment in bone marrow (B) and spleen (D). Each dot represents 1 mouse. Composite data from 3 independent experiments are shown. Numbers in plots represent frequencies within gates.