Flt3L treatment also augments ILC type 2 cells in reconstituted BRGSF mice. (A) Representative flow cytometry analysis of GATA-3⁺ ILCs in lung of reconstituted BRGSF mice treated or not with Flt3L. (B) Frequency among hCD45⁺ cells and total number of GATA-3⁺ ILCs in lung of reconstituted BRGSF mice treated or not with Flt3L. Composite data from 8 mice of 2 experiments is shown. (C) Representative functional analysis by IL-13 intracellular flow cytometry from freshly isolated lung ILCs, after P/I ex vivo stimulation, and after in vivo hydrodynamic cytokine injection and ex vivo stimulation. (D) Expression of CRTh2, CCR6, CD56, and CD117 (histograms) on lung GATA-3 ILCs (red) and EOMES⁺TBET⁺ NK cells (gray) assessed by flow cytometry. Each dot represents 1 mouse. Numbers in plots represent frequencies within gates. Max, maximum; w/o, without.