ILC3 can be found in the gut of BRGSF-reconstituted mice and their frequency increase after Flt3L treatment. (A) Total human CD45 cell number in gut of BRGSF mice treated or not with Flt3L. Composite data from of 12 to 16 mice per group. (B) Representative flow cytometry analysis of ILCs (CD3⁻CD5⁻CD94⁻CD7⁺CD127⁺) in gut of reconstituted BRGSF mice treated or not with Flt3L. (C) Frequency among hCD45⁺ cells and total number of ILCs in total gut of reconstituted BRGSF treated or not with Flt3L. Composite data from 6/8 mice of 2 experiments is shown. Each dot represents 1 mouse. (D) ILC (CD3⁻CD5⁻CD94⁻CD7⁺CD127⁺) (red) expression of CD117 and NKp46 as compared with NK cells (gray). (E) Intracellular expression of RORγt in gut ILCs of a representative Flt3L-treated mouse and the corresponding quantification (top) and representative functional analysis by IL-22 and IL-17 intracellular flow cytometry from ex vivo–stimulated ILCs. (F) Quantification of IL-22 and IL-17 production analyzed by flow cytometry from CD3⁻CD5⁻CD94⁻CD7⁺CD127⁺ ILC3 cells in response to TLR-mediated in vivo stimulation. Composite data from of 3 to 4 mice per condition are shown. Numbers in plots represent frequencies within gates.