Zbtb7a is downregulated in the absence of KLF1. Transcript levels of Zbtb7a were determined by quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) in Klf1^+/+ and Klf1^−/− fetal livers at E13.5 (A) and E14.5 (B). In each instance, Klf1^−/− samples were set to 1 (n = 5 per genotype). (C) Representative western blot of ZBTB7A expression in nuclear extracts isolated from fetal liver at E14.5. β-actin is presented as a loading control. (D) Transcript levels of Zbtb7a were determined by quantitative real-time RT-PCR in K1ER cells induced with tamoxifen for 0, 2, 4, 6, 8, 24, and 48 hours. The 0 hour time point was set to 1 (n = 2 per condition). (E) Representative western blot showing KLF1 and ZBTB7A expression in nuclear extracts isolated from K1ER cells at various time points postinduction. β-actin is presented as a loading control. (F) Transcript levels of Zbtb7a were determined by quantitative real-time RT-PCR in K1ER cells induced with tamoxifen for 0, 0.25, 0.5, 1, 2, 3, 4, and 48 hours. The 0 hour time point was set to 1 (n = 4). (G) K1ER cells were treated with either cycloheximide (CHX), tamoxifen, or a combination of both and harvested at 0, 2, 4, and 6 hours posttreatment. CHX was added to the appropriate cells 30 minutes before commencement induction with tamoxifen (n = 4 for each treatment). All RT-PCR values were normalized to 18S ribosomal RNA. Error bars represent the standard error of the mean. *P < .05; **P < .01 (paired Student 2-tailed t test).