Phenotype of leukocyte cone–derived ILC populations (A) Gating strategy used to purify ILC1s, ILC2s, and ILC3s from peripheral blood, in comparison with fluorescence minus one control controls, following pre-enrichment with NK combo protocol. Dot plots are representative of >10 independent experiments (n = 2 donors/experiment). (B) Differential expression of CD161 across the 3 ILC populations as depicted by a representative histogram. (C) Graph representative of the percentages of CD161⁺ and CD161⁻ populations for each of the ILC types. Data are shown as mean of the population; each dot represents 1 donor. FITC, fluorescein isothiocyanate; FMO, fluorescence minus one; FSC, forward scatter; SSC, side scatter.