Survival and proliferation of ILC2s. Purified ILC2s were stimulated with different cytokine cocktails for 5 days, and their survival and proliferation were evaluated by flow cytometry. (A) Percentage of survival, as defined by exclusion of the DAPI dye was compared in cells cultured in either IMDM or SFEM II media. (B) Heat map representing the average ILC2 survival between the 2 baseline media in all stimulation cocktails, where red represent highest expression and blue lowest expression. (C) Bar graph representing each individual replicate. (D-E) ILC2 cell number, following activation with EC alone, in double or triple combination in IMDM (D) or SFEM II media (E). Numbers above each treatment group represent fold change of proliferation above media control. Data are shown as mean ± standard error of the mean (SEM) from 3 independent experiments, with n = 2/3 donors each. Significance was calculated using 1-way ANOVA followed by correction for multiple comparisons, where *P < .05, **P < .01, ***P < .001, and ****P < .0001. Statistics above bars represent comparison with media baseline; comparison between samples is represented by the connecting line. ns, not significant.