ECs differentially modulate the expression of ILC2 canonical markers. ILC2s were stimulated in vitro with ECs or EC combinations, and surface expression of CD127, CRTH2, CD161, and CD117 was evaluated by flow cytometry. (A) Percentage of CD117⁺CD161⁺ in IMDM. (B) Contour plots showing the expression pattern of CD161 (x-axis) and CD117 (y-axis) after stimulation with double or triple cytokine combinations. (C-D) Mean fluorescence intensity (MFI) was also calculated for CD127 (C) and CRTH2 (D) in IMDM. Data are shown as mean ± SEM from 2 independent experiments, with n = 2 or 3 donors each. Significance was calculated using 1-way ANOVA followed by correction for multiple comparisons, where *P < .05, **P < .01, ***P < .001, and ****P < .0001. Statistics above bars represent comparison with media baseline; comparison between samples is represented by the connecting line. ns, not significant.