Molecular genetic responses of fit and senescent MSCs to IFNγ. Fit and senescent MSC pairs derived from 2 independent donors were stimulated with 20 ng/mL IFNγ for 48 hours, and total cDNA were generated from RNA. Transcriptional profiles of >40 genes were investigated in Fluidigm nanoscale qPCR 48*48 array plates (n = 3/sample). Heat map of fit (F) and senescent (S) MSCs stimulated with ±20 ng/mL IFNγ showing the expression genes (red = high expression, blue = low expression). Heat map was generated using JMP software.