Figure 6.
Figure 6. miR-125b regulates ETS1 and CBFβ genes critical for T-cell differentiation. (A-D) ETS1 and CBFβ expression negatively correlates with TLX3 and miR-125b expression in T-ALLs and T-cell progenitors. (A) Relative ETS1 messenger RNA (mRNA) levels in TLX3/OE (n = 6) and miR-125b/OE (n = 7) HPC-derived T cells at day 42 of culture. (B) Relative ETS1 mRNA levels in TLX3/KD DND41 cell line (n = 19) and primary T-ALL TLX3+ (n = 3). (C) Relative CBFβ mRNA levels in TLX3/OE (n = 3) and miR-125b/OE (n = 7) HPC-derived T cells at day 42 of culture. (D) Relative CBFβ mRNA levels in TLX3/KD DND41 cell line (n = 4) and primary T-ALL TLX3+ (n = 5). (E-F) ETS1 (E) and CBFβ (F) levels in TLX3− compared with TLX3+ T-ALLs (respectively, n = 30 vs n = 20 for ETS1 and n = 21 vs n = 16 for CBFβ). Shown are relative mRNA expressions normalized to ABL housekeeping gene (2−∆Ct). (A-F) Data are presented as mean ± SEM of values. *P < .05; **P < .01, Mann-Whitney U test.

miR-125b regulates ETS1 and CBFβ genes critical for T-cell differentiation. (A-D) ETS1 and CBFβ expression negatively correlates with TLX3 and miR-125b expression in T-ALLs and T-cell progenitors. (A) Relative ETS1 messenger RNA (mRNA) levels in TLX3/OE (n = 6) and miR-125b/OE (n = 7) HPC-derived T cells at day 42 of culture. (B) Relative ETS1 mRNA levels in TLX3/KD DND41 cell line (n = 19) and primary T-ALL TLX3+ (n = 3). (C) Relative CBFβ mRNA levels in TLX3/OE (n = 3) and miR-125b/OE (n = 7) HPC-derived T cells at day 42 of culture. (D) Relative CBFβ mRNA levels in TLX3/KD DND41 cell line (n = 4) and primary T-ALL TLX3+ (n = 5). (E-F) ETS1 (E) and CBFβ (F) levels in TLX3− compared with TLX3+ T-ALLs (respectively, n = 30 vs n = 20 for ETS1 and n = 21 vs n = 16 for CBFβ). Shown are relative mRNA expressions normalized to ABL housekeeping gene (2−∆Ct). (A-F) Data are presented as mean ± SEM of values. *P < .05; **P < .01, Mann-Whitney U test.

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