p-cMET and HGF protein levels vary in HUVECs cocultured with HR-MM cells. (A) Western blots showing the expression of cMET and HGF in MM cells (RPMI8226 and RPMI8226-HR) and HUVECs. (B) The intensities of cMET and HGF bands in panel A were quantified and normalized to the levels of β-actin (*P < .01 vs RPMI8226, Student t test, n = 3). (C) Schematic of the nonadherent coculture system of MM cells (blue) and HUVECs (green). MM cells and HUVECs were cocultured separately using a Transwell filter (polycarbonate membrane insert, 0.45-µm pores). (D) cMET, p-cMET, and HGF levels in HUVECs were measured by Western blotting after coculture with RPMI8226 or RPMI8226-HR cells under hypoxic conditions (1% O₂). (E) The intensities of cMET, p-cMET, and HGF bands in panel D were quantified and normalized to the levels of β-actin (*P < .01; **P < .001 vs control (HUVECs only), Student t test, n = 3).