Engraftment of CMML CD34⁺cells in NSG and NSGS mice. (A) General experimental procedure for engraftment. CD34⁺ cells were sorted from PB or BM samples collected from CMML patients using the MACS system (Miltenyi Biotec). One to 6 female mice, 6 to 8 weeks old, were irradiated at 2.5 Gy with a radiograph irradiator and IV injected within 24 hours with CD34⁺ cells suspended in 200 µL PBS. Human CD45⁺CD33⁺ cells were identified by flow cytometry in mouse blood samples collected at indicated time points postengraftment into NSG (B) or NSGS (D, left) or both (D, right) mice and in mouse BM samples taken from NSG (C) or NSGS (E) or both (F) mice when euthanized, 12 to 16 weeks after transplantation of cells from indicated samples. Each circle/square represents an individual mouse. “S” indicates the sample number. NSG mice are indicated by empty blue circles and NSGS mice by empty red squares.