American Society of Hematology

Figure 3.

$Figure 3. Multiparameter flow cytometry analysis of hCD45+ cells in the BM of engrafted mice. (A) Representative dot plot of flow cytometry analysis for immunophenotype of BM from NSG and NSGS mice xenografted from S944. The numbers listed represent the percent of human cells within gates P1 and P2 shown in left panel. (B) Percentage of human hCD45+ cells (left) within gates P1 and P2 calculated from NSG (11 patients) and NSGS (8 patients) primary mice 12 to 16 weeks posttransplantation. Each circle/square represents the mean percentage of human cells in different mice reconstituted with the same patient. Indicated fraction of cells among hCD45+ cells (right panel) in BM samples collected. No statistical differences were found in the percentages of cells expressing the different markers including CD34, CD15, and CD14 (Mann-Whitney U test). (C) Cytomorphology of human cells sorted from BM of NSG (left panels) and NSGS (right panels) recipient mouse xenografted from patient (S994). Black arrows indicate promonocytes and monoblasts; red arrows indicate myeloblasts. Original magnification ×500 (top panels) and ×1000 (bottom panels). (D) Genetic variants whose allele frequency (VAF) was measured in patient cells before and after engraftment in an NSGS mouse. Each dot represents 1 given mutation among screened genes.$

Multiparameter flow cytometry analysis of hCD45⁺cells in the BM of engrafted mice. (A) Representative dot plot of flow cytometry analysis for immunophenotype of BM from NSG and NSGS mice xenografted from S944. The numbers listed represent the percent of human cells within gates P1 and P2 shown in left panel. (B) Percentage of human hCD45+ cells (left) within gates P1 and P2 calculated from NSG (11 patients) and NSGS (8 patients) primary mice 12 to 16 weeks posttransplantation. Each circle/square represents the mean percentage of human cells in different mice reconstituted with the same patient. Indicated fraction of cells among hCD45⁺ cells (right panel) in BM samples collected. No statistical differences were found in the percentages of cells expressing the different markers including CD34, CD15, and CD14 (Mann-Whitney U test). (C) Cytomorphology of human cells sorted from BM of NSG (left panels) and NSGS (right panels) recipient mouse xenografted from patient (S994). Black arrows indicate promonocytes and monoblasts; red arrows indicate myeloblasts. Original magnification ×500 (top panels) and ×1000 (bottom panels). (D) Genetic variants whose allele frequency (VAF) was measured in patient cells before and after engraftment in an NSGS mouse. Each dot represents 1 given mutation among screened genes.

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