Secondary engraftment of CMML cells. (A) General experimental procedure for engraftment. Human cells were sorted from BM samples collected from primary mice and injected to secondary NSG or NSGS mice. (B) Comparison of secondary transplantation of NSG (green and purple circles) and NSGS (red and blue squares) mice. Multiparametric flow cytometry was used to identify the immunophenotype of human cells (C) and representative dot plots of flow cytometry of BM cells from primary and secondary recipient NSGS mice xenografted from S788 (D).