Chromatin remodeling and enrichment of DNA cis-elements in the promoter regions upon HSC differentiation to MPPs. (A) Heat map of reads density around gene promoters displaying an open chromatin state (from −1000 bp to 1000 bp) in LT-HSCs, ST-HSCs and MPPs, respectively. (B) The average reads density (smoothed) around gene promoters displaying an open chromatin state (from −1000 bp to 1000 bp) in LT-HSCs, ST-HSCs, and MPPs, respectively. (C) Heat map of significant enrichment value (-log [P value]) of transcription factor–binding motifs in gene promoters displaying an open chromatin state. (D) PCA for the enriched DNA motifs in open promoters within the 3 cell types. The first 2 components account for 90.67% and 9.33% of the total variance in the dataset, respectively. Dots represent the PCA loadings for the 166 transcription factors. The different colored dots represent 3 different cohorts. The motif of Krüppel-like factors (Klf9/Klf10) is displayed (top left). (E) Gene expression activity of Klf9 and Klf10 in LT-HSCs, ST-HSCs, and MPPs. Gene Expression Commons (https://gexc.stanford.edu). Gene expression activity ranged from −100 (most inactive) to 100 (most active).