Platelets from DAPP1^−/−mice are hyperresponsive to collagen-driven functional responses. (A) Whole blood from WT or DAPP1^−/− (KO) mice was loaded with DIOC6 and flowed over collagen (1000 s⁻¹, 3 min) before fixation and imaging by confocal microscopy; (i) representative images of z-slices at indicated intervals relative to the thrombus base; (ii) histogram of surface coverage; (iii) histogram of thrombus height. n = 5 + standard error of the mean. (B) CRP-mediated platelet aggregation in WT and DAPP1^−/− mouse platelets; (i) representative aggregation trace; (ii) histogram of the percentage of aggregation in response to a range of indicated CRP concentrations. n = 6 + standard error of the mean. Statistical analyses were performed by using Student t tests (A) or 2-way analysis of variance with Bonferroni post-tests (B). *P < .05; **P < .001.