American Society of Hematology

Figure 4.

$Figure 4. Erythrophagocytosis is greatly enhanced after blocking the “do not eat me” signal CD47 on the opsonized RBCs in vitro. (A) CD47-SIRPα interaction was blocked on unopsonized and IgG-coated RBCs with F(ab′)2 CD47. Splenocytes and RBCs were incubated for an hour and the phagocytic fraction was obtained using MACS. Absolute amounts of phagocytic cells are shown (n = 7-10). (B) Cytospins of the phagocytic fraction using anti-D-opsonized RBCs with CD47 blocking. These images where blindly chosen and are representative for 5 different experiments (original magnification ×50; May-Grünwald Giemsa stain). (C) F(ab′)2 fragments of anti-CD47 do not significantly increase RBC phagocytosis by neutrophils. However, F(ab′)2 CD47 in combination with anti-RhD opsonization does significantly increase RBC phagocytosis (n = 7-10). Error bars denote the standard error of the mean. Asterisks represent highly significant differences (*P < .05, ****P < .0001).$

Erythrophagocytosis is greatly enhanced after blocking the “do not eat me” signal CD47 on the opsonized RBCs in vitro. (A) CD47-SIRPα interaction was blocked on unopsonized and IgG-coated RBCs with F(ab′)2 CD47. Splenocytes and RBCs were incubated for an hour and the phagocytic fraction was obtained using MACS. Absolute amounts of phagocytic cells are shown (n = 7-10). (B) Cytospins of the phagocytic fraction using anti-D-opsonized RBCs with CD47 blocking. These images where blindly chosen and are representative for 5 different experiments (original magnification ×50; May-Grünwald Giemsa stain). (C) F(ab′)2 fragments of anti-CD47 do not significantly increase RBC phagocytosis by neutrophils. However, F(ab′)2 CD47 in combination with anti-RhD opsonization does significantly increase RBC phagocytosis (n = 7-10). Error bars denote the standard error of the mean. Asterisks represent highly significant differences (*P < .05, ****P < .0001).

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