Figure 5.
Figure 5. The CD47-SIRPα interaction regulates neutrophil phagocytosis of syngeneic RBCs in vivo. (A-B) Neutrophil uptake of IgG-opsonized Wt or CD47-deficient RBCs in vivo. PKH26-labeled IgG-opsonized RBCs were injected IV into Wt recipient mice and neutrophils in blood or spleen were analyzed for RBC uptake at 90 minutes after injection using flow cytometry (n = 4-5). (C) Phagocytosis of equally IgG-opsonized CD47 Wt or CD47-deficient (CD47KO) RBCs by Wt or SIRPα mutant bone marrow neutrophils in vitro in the presence of 1 µM fMLF. Error bars denote the standard error of the mean. Asterisks represent highly significant differences (****P < .0001).

The CD47-SIRPα interaction regulates neutrophil phagocytosis of syngeneic RBCs in vivo. (A-B) Neutrophil uptake of IgG-opsonized Wt or CD47-deficient RBCs in vivo. PKH26-labeled IgG-opsonized RBCs were injected IV into Wt recipient mice and neutrophils in blood or spleen were analyzed for RBC uptake at 90 minutes after injection using flow cytometry (n = 4-5). (C) Phagocytosis of equally IgG-opsonized CD47 Wt or CD47-deficient (CD47KO) RBCs by Wt or SIRPα mutant bone marrow neutrophils in vitro in the presence of 1 µM fMLF. Error bars denote the standard error of the mean. Asterisks represent highly significant differences (****P < .0001).

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