Intestinal Heph is not essential for the enhancement of iron absorption after induction of acute hemolysis. Adult mice were injected with the hemolytic agent (PHZ) to stimulate erythropoiesis. Five days later, iron absorption and utilization were determined. Hb levels were measured from blood samples taken at euthanization (A). Iron (⁵⁹Fe) absorption (B) and tissue distribution (C-F) were quantified by well-established methods. Data are shown as box-and-whisker plots and were analyzed by 3-way ANOVA. No significant 3-way interactions were noted. A PHZ main effect was noted for all measured parameters (P < .0001 for all). A sex main effect was noted for ⁵⁹Fe absorption (P = .0068) and for iron accumulation in blood, liver, and bone (P < .011 for all), but no genotype main effects were observed for these parameters. PHZ × sex interactions were noted for blood (P = .0016) and spleen (P = .0107) ⁵⁹Fe accumulation, and a genotype × sex interaction was observed for ⁵⁹Fe absorption (P = .0419). The number of mice in each group is indicated in parentheses beneath panel A and is the same for the data shown in all panels, unless otherwise indicated.