Treatment with all 4 concentrations of UFH restored the network-formation abilities of DCN-reduced TIME cells. The ability of TIME cells to form networks after DCN siRNA or Mock/NC was determined using the μ-Slide Angiogenesis system by IBIDI. (A) Representative image of the networks stained with calcein-AM is shown for each concentration. Images were acquired using the Olympus BX53 fluorescence microscope and represent 10× magnification to obtain the whole field. (B) The network-formation potential of TIME cells after treatment with 4 concentrations of UFH calculated by the Wimasis software. *P < .05, **P < .001, ***P < .0001, ****P < .00001 (all significant); n = 9; 1-way ANOVA. The y-axis represents the number of branch points.