Treatment with all 4 concentrations of NAC restored the network-formation abilities of DCN-reduced TIME cells. (A) Representative image of the networks stained with calcein-AM is shown for each concentration. Images were acquired using the Olympus BX53 fluorescence microscope and represent 10× magnification to obtain the whole field. (B) Network formation of TIME cells after treatment with NAC calculated by the Wimasis software. *P < .05, **P < .001 (both significant); n = 9; 1-way ANOVA. The y-axis represents the number of branch points.