Multimodal regulation of LPS-induced WNT ligand expression by innate immune signaling pathways and inflammatory cytokines. Gene expression was determined in spleen tissue of mice injected with LPS (2 mg/kg) for 1.5 and 3 h. Mice deficient in TLR signaling adaptor proteins (A) MYD88 or (B) TRIF were compared with wild-type (WT) controls at each time point by 2-tailed Mann-Whitney U test. Values for individual mice analyzed in 3 independent experiments are depicted, and mean ± standard error of the mean are indicated. Mice deficient in the inflammatory cytokines (C) TNF and (D) IL-12/IL-23p40 were compared with WT controls in 2 independent experiments. Gene expression for individual mice is depicted, and means ± standard error of the mean are indicated. Groups were compared by 2-tailed Student t test. *P < .05, **P < .01, ****P < .0001, n.s. not significant. KO, knockout.