Figure 2.
Fibrin induces Syk-dependent platelet activation and aggregation. (A) Platelets (500 × 109/L), pretreated where indicated with dasatinib (dasat; 3 μM) or PRT-060318 (10 μM), were stimulated with fibrin dispersed as a suspension. (B) The histogram represents 3 independent experiments. The results are shown as mean ± standard deviation. **P < .01. (C) Stimulations were stopped with addition of 2× lysis buffer. A sample of the whole-cell lysate (WCL) was removed, and the remaining lysate was used to immunoprecipitate (IP) Syk. WCLs and IPs were separated by SDS-polyacrylamide gel electrophoresis and western blotted (WB) for pTyr and Syk. The results are shown as a representative of 3 independent experiments.
