Figure 2.
![Figure 2. 3D reconstruction of labeled platelets. Z series of images of platelets shown in Figure 1 were subjected to 3D rendering to emphasize the distinct distribution of puncta labeled by DQ BSA (A; red) or LysoTracker Red DND-99 (B; red) from those labeled by mepacrine in 3D space. (A) Platelet from the first row of Figure 1A is shown in 2 views (view 1: 169°, −12°, −169° [X, Y, Z]; view 2: 8°, −12°, 141°). (B) Platelet from the first row of Figure 1B is shown in 2 views (view 1: −7°, 3°, 17°; view 2: −172°, −3°, 112°). The x- and y-axes are labeled for scale in micrometers. Three-dimensional reconstruction was performed with Leica Application Suite software.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/1/19/10.1182_bloodadvances.2017006726/3/advances006726f2.jpeg?Expires=1735799846&Signature=rWQKjwpDtoN8wMAvazvZVA2DNR~y1~09clkf1wi302r1P-YicxcrQV8e-0p~m6xkkUFlRoyPxh7wt70csmzMlgnuZSqKIGgTvlTp86fuVf2U3576ohpTMc2GopLewVWgUT-orSGhbLFLJ0bmi1L7i1VfOSRm7O701A7zApXy28ej8RIYFeh53JtqmNQ-6538NtGdOkWUFmsV7sIff7zkiW7kUSFeQwyuYWzqN2O9WLEUFGcGZrc76cVl5UHUkhsl2QFJ10UM1lloMlMi5OLCqO~2YQ-FCUCEGfqmKStVRQ1L~WRdwCGe4fqDBNsNCaGZUcVZ7m6NCcx60Z8bFOyWWQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
3D reconstruction of labeled platelets.Z series of images of platelets shown in Figure 1 were subjected to 3D rendering to emphasize the distinct distribution of puncta labeled by DQ BSA (A; red) or LysoTracker Red DND-99 (B; red) from those labeled by mepacrine in 3D space. (A) Platelet from the first row of Figure 1A is shown in 2 views (view 1: 169°, −12°, −169° [X, Y, Z]; view 2: 8°, −12°, 141°). (B) Platelet from the first row of Figure 1B is shown in 2 views (view 1: −7°, 3°, 17°; view 2: −172°, −3°, 112°). The x- and y-axes are labeled for scale in micrometers. Three-dimensional reconstruction was performed with Leica Application Suite software.
