Immunohistochemical evaluation of hedgehog signaling pathway molecules. (A) Mean H-scores for hedgehog signaling pathway molecules obtained from pre- and posttreatment skin biopsies from 13 evaluable patients. The H-score is calculated by multiplying the percentage of cells staining for a given molecule in a fixed field by the staining intensity (0 = none, 1 = weak, 2 = moderate, 3 = intense), with final scores ranging from 0 to 300. (B) Histochemical staining demonstrating a decrease in staining intensity for hedgehog pathway molecules in skin biopsies from a sample clinical responder. Hematoxylin and eosin (H&E) stain, original magnification ×600; all other stains, original magnification ×200. GSK3β, glycogen synthase kinase 3 β; Ihh, Indian hedgehog; Shh, sonic hedgehog.