HSP70 expression level and subcellular localization measured by imaging flow cytometry (ImageStream) in human erythroid cells from various DBA patients and after RPS19 or RPL11 or RPL5 depletion of primitive CD34⁺cord blood cells. (A) HSP70 expression level measured by imaging flow cytometry (ImageStream) into the cytoplasm (top panels) and nucleus (bottom panels) in erythroid cells from DBA-affected patients (RPS19^+/Mut UPN#37, RPL11^+/Mut UPN#189 and UPN#1130 patients) and relatives (Co mother and father) at day 7 of culture compare with healthy controls (Co). Results are presented as the mean fluorescence intensity (MFI). (B) HSP70 expression analysis compared with actin in the shRNA model using imaging flow cytometry in the total erythroid cells and in the cytoplasm and nucleus of these cells (ImageStream). The data are shown as the mean ± the standard deviation of 4 independent experiments. RPL5 shown as another non RPS19 gene. ****P < .0001.