Figure 1.
Figure 1. rhF8 dose response on FVIII-sensitized CD4+ T-cell proliferation. Splenocytes isolated from rhF8-immunized FVIIInull mice were labeled with CellTrace Violet and cultured with various doses of rhF8 or rhVWF for 96 hours. Concanavalin A was used as a positive control for T-cell proliferation. Cells were stained with anti–mouse CD4 and anti–mouse TCRβ antibodies and analyzed by flow cytometry for daughter CD4+ T cells. (A) Representative flow cytometry histograms. (B) rhF8 dose–response curve.

rhF8 dose response on FVIII-sensitized CD4+T-cell proliferation. Splenocytes isolated from rhF8-immunized FVIIInull mice were labeled with CellTrace Violet and cultured with various doses of rhF8 or rhVWF for 96 hours. Concanavalin A was used as a positive control for T-cell proliferation. Cells were stained with anti–mouse CD4 and anti–mouse TCRβ antibodies and analyzed by flow cytometry for daughter CD4+ T cells. (A) Representative flow cytometry histograms. (B) rhF8 dose–response curve.

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