Cytokine profile analysis. Splenocytes isolated from rhF8-immunized FVIII^null mice were cultured with rhF8 with or without rhVWF for 96 hours. The conditioned media was used to analyze the cytokine expression using the Bio-Plex cytokine assay. (Ai-Aii) Impact of VWF on IFN-γ expression in rhF8-sensitized splenocytes in response to rhF8 restimulation. (Bi-Bii) Impact of VWF on IL-10 expression in rhF8-sensitized splenocytes in response to rhF8 restimulation. The paired Student t test was used to compare data sets. These data demonstrate that VWF can attenuate IFN-γ production in FVIII-sensitized splenocytes in response to rhF8 restimulation.