Hierarchical relationships of normal HSPCs are not maintained in BP-CML. (A) Percentage purities of population, after FACS sorting, injected for secondary transplant (Tx) from 2 patients (COL091, CML002). (B) Secondary engraftment of cells from 2 patients (COL091 and CML002). x-axis: bottom: patient sample populations injected into primary mice; top: population from primary engrafted mouse injected into secondary recipient. y-axis: mean percentage human (h)CD45⁺CD33⁺CD19⁻ cell engraftment/total live MNC. Red line: engraftment threshold. Each dot represents 1 injected mouse: black dots: engrafted mice; red dots: mice that failed to engraft.