Figure 3.
Oxidase activity following Cre excision. Analysis of DHR oxidase activity in myeloid differentiated iPSC lines following Cre-mediated recombination excision of the puromycin cassette. Shown are analyses of corrected iPSC lines (P47-04C2,4,5-Cre GT exon 2 corrected; P47-04-C1M-Cre minigene corrected) compared with uncorrected (P47-04), healthy control iPSCs, or healthy control peripheral blood. The DHR assay, gating for MFI assessment, and donor target assessment are as described in the Figure 2D legend.