Analysis of HIV receptors expressed by PB-CD34 cells and modulated by latent HCMV. Mock or HCMV strain Merlin was used for infection of PB-CD34 cells from 2 independent donors. On day 5 PI, cells were immunostained with isotype or specific antibodies (Abs) for (A) CXCR4 and CCR5, with subgating to illustrate coexpression with frequencies depicted for 2 donors (right) analyzed by flow cytometry. (B) CD4 expression on PB-CD34 cells were analyzed by flow cytometry based on isotype controls. (C) Agarose gel electrophoresis of RT-PCR products performed for CD4, HCMV US28, and GAPDH. Arrows point to the expected product size. Ladder, 100-bp DNA ladder; Neg, no template control.