TA-316 induces megakaryopoietic differentiation from iPS-Sac–derived hematopoietic progenitor cells. (A) Scheme of megakaryopoiesis and thrombopoiesis in iPS-Sac–derived iHPCs. hiPSCs cultured for 14 days with VEGF-formed sac-like structures (iPS-Sacs). CD43⁺/CD34⁺/CD41⁻/GPA⁻ iHPCs were sorted from crude iHPCs in iPSC-Sacs using FACS and then cultured with rhTPO (100 ng/mL), TA-316 (100 ng/mL), or eltrombopag (3000 ng/mL). (B) The number of CD41⁺ cells was evaluated using FACS on days 3 and 5. Data represent the mean and SD; **P < .01, ***P < .001. n.s., not significant.