TA-316 promotes megakaryopoiesis and thrombopoiesis in imMKCLs. (A) System to generate platelets from imMKCLs cultured with rhTPO, TA-316, eltrombopag, or DMSO (vehicle). Cells were seeded onto mitomycin C-treated C3H10T1/2 feeder cells and passaged every 3 to 4 days. To turn off the c-MYC, BMI1, and BCL-XL genes in order to induce platelet production, beginning on day 11, the cells were cultured in DOX-free medium. After 4 days of culture, matured MKs and platelets were collected. (B) The number of imMKCLs was counted on day 11 based on Trypan blue assays. (C) On day 15, imMKCL-derived platelets were collected, and CD41⁺/CD42b⁺ platelets were counted using FACS. Data represent the mean and SD; **P < .01, ***P < .001.